羅元超 博士

研究副技師

中央研究院 生醫轉譯研究中心 藥物合成及分析核心設施-核磁共振

Office: 轉譯中心 B743 (國家生技研究園區)

Office TEL: (02)7750-5811

Lab TEL: (02)7750-5500 #2007

E-mail: yclou@gate.sinica.edu.tw  

 

 

1996-2002 博士 國立清華大學 生命科學系

1992-1996 學士 國立清華大學 化學工程系

 

 

2024 ~ 迄今 研究副技師，中央研究院 生醫轉譯研究中心
2018 ~ 迄今 設施經理，藥物合成及分析核心設施-核磁共振
2020 ~ 迄今 理事，台灣磁共振學會
2021 ~ 2024 專案研究人員，中央研究院 生醫轉譯研究中心
2019 ~ 2021 特殊性技術人員，中央研究院 生醫轉譯研究中心
2018 ~ 2019 博士後研究員，中央研究院 化學研究所
2016 ~ 2018 秘書長，台灣磁共振學會
2002 ~ 2018 博士後研究員，中央研究院 生物醫學科學研究所

 

●　小分子及生物巨分子液態核磁共振光譜學

●　生物物理及結構生物學

●　代謝體學

 

 

2024    中央研究院新聘學術研究獎金
2014    第十五屆國際生物化學與分子生物學聯合會（IUBMB；台灣），傑出壁報論文獎
2010    歐洲磁共振大會與第十七屆國際磁共振協會聯合會議(義大利)，旅行獎助
2006    傑出人才發展基金會，優秀學生旅行獎助
2005    第一屆亞太區核磁共振研討會(日本)，青年科學家獎
1999    第二屆台日核磁共振研討會(台灣)，傑出壁報論文獎

 

 

1. Štěrbová P, Wang CH, Carillo KJD, Lou YC, Kato T, Namba K, Tzou DM, Chang WH. “Molecular Mechanism of pH-Induced Protrusion Configuration Switching in Piscine Betanodavirus Implies a Novel Antiviral Strategy.” (2024) ACS Infect. Dis. 10(9):3304-3319
2. Lou YC, Tu CF, Chou CC, Yeh HH, Chien CY, Sadotra S, Chen C, Yang RB, Hsu CH. “Structural insights into the role of N-terminal integrity in PhoSL for core-fucosylated N-glycan recognition” (2024) Int. J. Biol. Macromol. 255:128309 
3. Lou YC, Huang HY, Yeh HH, Chiang WH, Chen C, Wu KP. “Structural basis of transcriptional activation by the OmpR/PhoB-family response regulator PmrA” (2023) Nucleic Acids Res. 51(18):10049-10058
4. Yu CL, Chuang TW, Samuel SY, Lou YC, Tarn WY. “Co-phase separation of Y14 and RNA in vitro and its implication for DNA repair” (2023) RNA 29(7):1007-1019
5. Kumar JV, Tseng TS, Lou YC, Wei SY, Wu TH, Tang HC, Chiu YC, Hsu CH, Chen C. “Structural insights into DNA binding domain of vancomycin-resistance-associated response regulator in complex with its promoter DNA from Staphylococcus aureus” (2022) Protein Sci. 31(5):e4286.
6. Wu D, Lou YC, Chang W, Tzou DM. “NMR assignments of vaccinia virus protein A28: an entry-fusion complex component” (2021) Biomol NMR Assign. 15(1):117-120.
7. Sadotra S, Lou YC, Tang HC, Chiu YC, Hsu CH, Chen C. “Structural basis for promoter DNA recognition by the response regulator OmpR” (2021) J Struct Biol. 213(1):107638
8. Lee CH, Lou YC, Wang AH. “DMTMM-Mediated Intramolecular Cyclization of Acidic Residues in Peptides/Proteins” (2021) ACS Omega. 6(7):4708-4718
9. Aryal S, Hsu HM, Lou YC, Chu CH, Tai JH, Hsu CH, Chen C. “N-Terminal Segment of TvCyP2 Cyclophilin from Trichomonas vaginalis Is Involved in Self-Association, Membrane Interaction, and Subcellular Localization.” (2020) Biomolecules. 10(9):1239
10. Lan YJ, Yeh PS, Kao TY, Lo YC, Sue SC, Chen YW, Hwang DW, Chiang YW. “Anti-apoptotic BCL-2 regulation by changes in dynamics of its long unstructured loop.” (2020) Commun Biol. 3(1):668
11. Bosken YK, Cholko T, Lou YC, Wu KP, Chang CA. “Insights into Dynamics of Inhibitor and Ubiquitin-like Protein Binding in SARS-CoV-2 Papain-like Protease” (2020) Frontiers in Molecular Biosciences. 7:174
12. Štěrbová P, Wu D, Lou YC, Wang CH, Chang WH, Tzou DM. “NMR assignments of protrusion domain of capsid protein from dragon grouper nervous necrosis virus” (2020) Biomol NMR Assign. 14(1):63-66
13. Lou YC, Chou CC, Yeh HH, Chien CY, Sadotra S, Hsu CH, Chen C. “Structural basis for -35 element recognition by σ4 chimera proteins and their interactions with PmrA response regulator” (2020) Proteins 88(1):69-81
14. T. Martin, Lou YC, C.C. Chou, S.Y. Wei, S. Sadotra, C.C. Cho, M.H. Lin, J.H. Tai, C.H. Hsu and C. Chen. "Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis" (2018) Scientific Reports 8(1):5410.
15. T. Martin, Lou YC, S. Aryal, J.H. Tai and C. Chen. "1H, 13C and 15N resonance assignments and secondary structures of cyclophilin 2 from Trichomonas vaginalis" (2018) Biomolecular NMR Assignments. 12(1):27-30 
16. C.C. Chou, S.Y. Wei, Lou YC and C. Chen. "In-depth study of DNA binding of Cys2His2 finger domains in testis zinc-finger protein" (2017) PLoS One. 12(4):e0175051.
17. T.W. Chuang, K.M. Lee, Lou YC, C.C. Lu, W.Y. Tarn “A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement” (2016) Journal of Biological Chemistry. 291(16):8565-74. 
18. Lou YC, T.H. Weng, Y.C. Li, Y.F. Kao, W.F. Lin, H.L. Peng, S.H. Chou, C.D. Hsiao and C. Chen “Structure and dynamics of polymyxin-resistance-associated response regulator PmrA in complex with promoter DNA” (2015) Nature Communications. 13(6):8838. 
19. Lou YC, Y.F. Kao, K.H. Chin, J.K. Chen, J.L. Tu, C. Chen and S.H. Chou “Backbone resonance assignments of the 54 kDa dimeric C-terminal domain of murine STING in complex with DMXAA” (2015) Biomolecular NMR Assignments. 9(2):271-4
20. Lou YC, I. Wang, M. Rajasekaran, Y.F. Kao, M.R. Ho, S.T. Danny Hsu, S.H. Chou, S.H. Wu and C. Chen “Solution structure and tandem DNA recognition of the C-terminal effector domain of PmrA from Klebsiella pneumoniae” (2014) Nucleic Acids Research. 42(6):4080-93
21. S.C. Luo, Lou YC, M. Rajasekaran, Y.W. Chang, C.D. Hsiao and C. Chen “Structural Basis of a Physical Blockage Mechanism for the Interaction of Response Regulator PmrA with Connector Protein PmrD from Klesiella Pneumoniae.” (2013) Journal of Biological Chemistry. 288(35):25551-61
22. K.H. Chin, Z.L. Tu, Y.C. Su, Y.J. Yu, H.C. Chen, Lou YC, C.P. Chen, G.N. Barber, M.L. Chuah, Z.X. Liang and S.H. Chou “Novel c-di-GMP recognition modes of the mouse innate immune adaptor protein STING.” (2013) Acta Crystallographica Section D Biological Crystallography 69:352-66. 
23. Y.F. Kao, Lou YC, Y.H. Yeh, C.D. Hsiao, C.D. and C. Chen “Solution structure of the C-terminal NP-repeat domain of Tic40, a co-chaperone during protein import into chloroplasts” (2012) Journal of Biochemistry 152:443-51. 
24. S.Y. Wei, Lou YC, J.Y. Tsai, M.R. Ho, C.C. Chou, M. Rajasekaran, H.M. Hsu, J.H. Tai, C.D. Hsiao and C. Chen “Structure of the Trichomonas vaginalis Myb3 DNA-binding domain bound to a promoter sequence reveals a unique C-terminal -hairpin conformation” (2012) Nucleic Acids Research. 40(1):449-460. 
25. Y.R. Pan, Lou YC, J. Rizo, and C. Chen “NMR structure and calcium-binding properties of the tellurite resistance protein TerD from Klebsiella pneumoniae” (2011) Journal of Molecular Biology, 405(5):1188-201. 
26. M.R. Ho, Lou YC, S.Y. Wei, S.C. Luo, W.C. Lin, P.C. Lyu and C. Chen “Human Reg IV protein adopts a typical C-type lectin fold but binds mannan with two calcium-independent sites” (2010) Journal of Molecular Biology, 402(4):682-695. 
27. S.C. Luo, Lou YC, H.Y. Cheng, Y.R. Pan, H.L. Peng, and C. Chen “Solution structure and phospho-PmrA recognition mode of PmrD from Klebsiella pneumoniae.” (2010) Journal of Structural Biology, 172(3):319-330. 
28. C.C. Chou, Lou YC, T.K. Tang and C. Chen “Structure and DNA binding characteristics of the three-Cys2His2 domain of mouse testis zinc finger protein” (2010) PROTEINS: Structure, Function, and Bioinformatics. 78(10): 2202-12. 
29. Lou YC, S.Y. Wei, M. Rajasekaran, C.C. Chou, H.M. Hsu, J.H. Tai and C. Chen “NMR structural analysis of DNA recognition by a novel Myb1 DNA binding domain in the protozoan parasite Trichomonas vaginalis” (2009) Nucleic Acids Research. 37(7):2381-94.
30. S.K. Chiang, Lou YC, and C. Chen “NMR solution structure of KP-terB, a tellurite resistant protein from Klebsiella pneumoniae” (2008) Protein Science 17(4):785-789. 
31. I. Wang, Lou YC, Y.C. Lin, S.C. Lo, A. Y.L. Lee, S.H. Wu and C. Chen. (2007) “1H, 13C and 15N resonance assignments of α-domain for Bacillus subtilis Lon Protease” Biomolecular NMR Assignments 1(2):201-203. 
32. M.R. Ho, Lou YC, W.C Lin, P.C Lyu, W.N. Huang, and C. Chen “Human pancreatitis-associated protein forms fibrillar aggregates with a native-like conformation” (2006) Journal of Biological Chemistry 281(44):33566-76. 
33. Lou YC, Y.C. Huang, Y.R. Pan, C. Chen, and Y.D. Liao “Roles of N-terminal pyroglutamate in maintaining structural integrity and pKa values of catalytic histidine residues in bullfrog ribonuclease 3” (2006) Journal of Molecular Biology 355(3):409-21. 
34. Y.R. Pan, K.P. Wu, Lou YC, Y.D. Liao, and C. Chen “Letter to the Editor: 1H, 13C and 15N resonance assignments and secondary structure of murine angiogenin 4” (2005) Journal of Biomolecular NMR 31(2):175-6. 
35. Iren Wang, Lou YC, K.P. Wu, S.H. Wu, W.C. Chang, and C. Chen “Novel solution structure of porcine -microseminoprotein” (2005) Journal of Molecular Biology 346(4):1071-1082. 
36. M.R. Ho, Lou YC, W.C. Lin, P.C. Lyu, and C. Chen “Letter to the Editor: 1H, 13C and 15N resonance assignments and secondary structure of human pancreatitis- associated protein (hPAP)” (2004) Journal of Biomolecular NMR 30(3):381-2. 
37. K.P. Wu, C.W. Wu, Y.P. Tsao, T.W. Kuo, Lou YC, C.W. Lin, S.C. Wu, and J.W. Cheng “Structural basis of a flavivirus recognized by its neutralizing antibody: solution structure of the domain III of the Japanese encephalitis virus envelope protein.” (2003) Journal of Biological Chemistry 278(46):46007-13. 
38. Lou YC, Y.R. Pan, Y.H. Ho, Y.D. Liao, and C. Chen “Letter to the Editor: 1H, 13C and 15N resonance assignments and secondary structure of the cytotoxic RNase 3 from oocytes of bullfrog Rana catesbeiana” (2003) Journal of Biomolecular NMR. 27(3):289-90. 
39. Lou YC, I.J. Lin, M.T. Pai, and J.W. Cheng "Solution Structure of an N-Capping Peptide from the N-terminal Leucine-Repeat Region of Hepatitis Delta Antigen" (2000) Archives of Biochemistry and Biophysics 377, 219-227. 
40. S.R. Tzeng, Lou YC, M.T. Pai, and J.W. Cheng "Solution Structure of the BTK SH3 Domain Complexed with a Proline-Rich Peptide from p120cbl" (2000) Journal of Biomolecular NMR 16, 303-312. 
41. Lou YC, F.T. Lung, M.T. Pai, S.R. Tzeng, P.P. Roller, and J.W. Cheng "Solution Structure and Dynamics of a Nonphosphorelated Cyclic Peptide Inhibitor for the Grb2 SH2 Domain" (1999) Archives of Biochemistry and Biophysics 372, 309-314. 
42. I.J. Lin, Lou YC, M.T. Pai, H.N. Wu, and J.W. Cheng "Solution Structure and RNA Binding Activity of the N-terminal Leucine-Repeat Region of Hepatitis Delta Antigen" (1999) PROTEINS: Structure, Function, and Genetics 37, 121-129. 
43. J.W. Cheng, I.J. Lin, Lou YC, M.T. Pai, and H.N. Wu "Local Helix Content and Nucleic Acid Binding Activity of the N-terminal Leucine-Repeat Region of Hepatitis Delta Antigen" (1998) Journal of Biomolecular NMR 12, 183-188. 

 

 

1. 羅元超、何孟儒和陳金榜“人類胰臟炎相關蛋白形成纖維化沈澱之機制” 中央研究院學術諮詢總會通訊，第十五卷第二期，中華民國96年3月
2. 羅世奇、羅元超、江盛國、潘韻如和陳金榜“克雷伯氏肺炎桿菌核磁共振結構基因體學研究”科儀新知150，第二十七卷第四期，中華民國95年2月
3. 羅元超和陳金榜 “NMR在蛋白質體學之研究”教育部顧問室生物技術科技教育改進計畫專書 蛋白質體學，第十七章，中華民國92年12月
4. 程家維、曾秀如、羅元超和白明道“高磁場核磁共振在循理式藥物設計上之應用”科儀新知110，第二十卷第六期，中華民國88年6月